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Sample Preparation Of HPLC Column
Sep 14, 2017

1, the sample should be dissolved as far as possible with the flow of the solvent soluble. In addition to special specified, the resolution of the column with strong solvent dissolution of the sample may be reduced.

2. The sample solution should be filtered beforehand using a needle-type filter before sampling. Frequent changes in the composition of the flow phase will accelerate the reduction of column efficiency.

3, chromatographic column by high-pressure homogenate filling, can withstand higher pressure. In order to obtain the best separation effect, please do not exceed 200kgf when using, and avoid the pressure suddenly rise or change, otherwise will cause the destruction of silica gel filler, reduce the service life of chromatographic column. The maximum operating temperature should not exceed 60 ℃ in addition to special requirements.


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